Posts

Showing posts with the label DNA-Protein Interaction Molecular Techniques

Featured post

ANATOMY OF FLOWERING PLANTS

-
Image
  What is Plant Anatomy? Plant anatomy is the study of internal structure and organization of tissues in plants. It helps in understanding: Functional adaptation Transport system Growth patterns Tissue Organization in Flowering Plants Flowering plants have three major tissue systems :  Epidermal Tissue System Components: Epidermis Single layer of compact cells No intercellular spaces Covered by cuticle (except roots) Cuticle Made of cutin Prevents water loss Stomata Present mainly on leaves Composed of guard cells Regulate: Gas exchange Transpiration Root hairs Extensions of epidermal cells Increase surface area for absorption Trichomes (in stem) Hair-like structures Protection + reduce transpiration  Functions: Protection Water conservation Gas exchange 🌿 B. Ground Tissue System  Types of Ground Tissue: 1. Parenchyma Living cells, thin cell wall Large vacuole Functions: Storage Photosynthesis ( chlorenchyma ) Air storage ( aerenchyma ) 2. Collenchyma Living cell...
Post a Comment
Read more

DNA-Protein Interaction Molecular Techniques

-
Image
Electrophoretic Mobility Shift Assay (EMSA) Design and preparation of DNA probes: Determine the DNA sequence of interest and design short DNA probes (typically 20-50 base pairs) containing the putative binding sites for the protein under study. Synthesize the DNA probes or obtain them commercially. Label the probes with a suitable marker, such as a radioisotope (e.g., [32P]) or a fluorescent dye. The label is usually attached to either the 5' or 3' end of the DNA probe. Protein extraction and purification: Extract the protein of interest from the desired cellular or tissue source using appropriate methods. This may involve cell lysis, fractionation, or purification steps. Purify the protein using techniques like chromatography to obtain a more concentrated and homogeneous protein sample. Binding reaction: Prepare a binding reaction mixture by combining the purified protein with the labeled DNA probe in a binding buffer. The binding buffer provides optimal conditions for the pro...
Post a Comment
Read more